| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: corticotropin-releasing factor receptor subtype 2 [CRF2R] Alleles: A6, B1, D2, A5, B2, C2, D1, A1, A2, A2, A1, B1, A1, A2, A3, A4, B2, C1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--16.5, 7.5 (doublet), 6.9, 2.55, 1.6; HindIII--14.0, 12.5, 8.6, 3.75, 2.95, 1.7; BglII--18.0, 13.5, 12.0; SalI--43.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
