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Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout

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編號:B233966

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產品名稱	Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout
商品貨號	B233966
Deposited As	Cryptococcus bacillisporus
Strain Designations	A1M R265 [CBS 10514]
Alternate State	Filobasidiella bacillispora Kwon-Chung, teleomorph
Application	Multigene phylogeny and phenotypic characterization. Biomedical Research and Development Material
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Antigenic Properties	Serotype B
Genotype	VGIIa, AFLP6A
Preceptrol®	no
Genome Sequenced Strain	Yes
Mating Type	alpha
Comments	Genome sequencing strain (Broad Institute, USA) Cause cryptococcosis Represents one of the two genotypes causing an outbreak in Vancouver Island Multigene phylogeny and phenotypic characterization Designated as type strain of Cryptococcus deuterogattii Hagen & Boekhout by Hagen et al. (2015)
Medium	ATCC^® Medium 28: Emmons' modification of Sabouraud's agar ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 1245: YEPD
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC 26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAATATGTTAGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGAGGCTTTGCTTTTTTTTATGACGATGACTTGTCAAAGTGTGGATGACTCCAGGAGAGAGGTCGGGCTGGTAATTAGTAATATCAGTCAGTCATTTCAGCTGGCGCCATCGATACTTTATAAGTCAAACTTAACCATTTCACTGTGTAGTAAGTAGTAGGCTCTGAATTACTAGAGACACTTGCCAGGTTATCGCAAGTTGGCAGGCAGGCAGGACACACATACTATTGATTTCACGGTCATGGGGGACTTGGCTGCTGGTGCTTGAGTTGCATAATAGAGAGAGTATATGCATGTCGCGGGGGGGGGACTTGGCTAAGATGCGTTATGCGGCAAGCAAGGTAAGTAGTATTTGTATTAAAGTATTTAGTTACTTTTCCAGTCATGGGGGACTTTGATAGTGGTTGTGAGCCGGAATGAAAAATGGGTAAATGTGGTATGGATGGTGAGAGGGGGAGCTGCCAAGTGTATGCAGTGGGTGTGCTTATAGCATAGTCAAGAGGGGATGGGCAATTAAATTTTGGAAAGTTGTTTGACCCGACCTGACGGTGACTCTAATAGGCTGGTCAAGCAAACGTTTAAGTTGAGTCAAGCTCGGTCAAGCAAAGTCTAGAAAAGATTAATGATCGCTAAGAATTACTCCGGTCGCGGGGGGCTTGCAACTTGTCTGGCCCACTGTGGGTCCAGTTT glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds TACTTGCATAAATACAATGGTTGTCAAGGTTGGAATCAACGGTTTCGGTACGTGATCTGTCTCTATCAAGCGTTGCTTCATATGTCACCAATGTGGTTTCCGATATTGTGCACCGTCCTTGGGTTACTGGTAGCTAATAGTTATGCGATAGGTCGTATCGGTCGAATTGTTCTCAGGTATGCTTTAAGTCAAGGGGTAAGACAGCTGCGTATCTAACCATCAAATTAGGAACGCCATCGAGCATGGAGACCTTGAGGTTGTTGCTGTCAACGAGTGAGTATTCTCAAAAGTGTTAATCTATTAAATAAATTATTTACTTTTGCAAAGCCCTTTCATTGACTTGGACTACATGGTATAATACTTTCTTCTGTACAATTGCTTCGTTTTGTGCTAACTGTAGCATGCGTAGGTTTACATGTTCAAGTACGACTCCGTAAGTCTCTGATGGCTCTAGCGCTCTATATGGCATTTTAACCTCATTCCCAACAGACACATGGTCGCTTCAAGGGTTCTGTCGAGGTTAAGGACGGTAAGCTCTATATCAACAACAAGGCCATTTCCGTTTTCGGTGAGAAGGACCCCGCCAACATCAAGTGGGGTGACGCTGGTGCCGAGTACATCGTTGAGTCTACCGGTGTCTTCACCACTACCGAAAAGGCCGGTGTCCAT
Name of Depositor	L Hoang
Special Collection	NSF - Mycology
Chain of Custody	ATCC <-- L. Hoang
Isolation	Bronchial alveolar lavage, Duncan, Vancouver Island, B.C. Canada.
Cross References	Nucleotide (GenBank) : AAFP00000000 Cryptococcus gattii VGII R265, whole genome shotgun sequencing project
References	Hu G, Kronstad JW. Gene disruption in Cryptococcus neoformans and Cryptococcus gattii by in vitro transposition. Curr Genet 49: 341-350, 2006. PubMed: 16397763 Fraser James A, et al. Same-sex mating and the origin of the Vancouver Island Cryptococcus gattii outbreak. Nature 437: 1360-1364, 2005. Kidd Sarah E, et al. Comparative gene genealogies indicate that two clonal lineages of Cryptococcus gattii in British Columbia resemble strains from other geographical areas. Eukaryot. Cell 4: 1629-1638, 2005. Kidd Sarah E, et al. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc. Natl. Acad. Sci. USA 101: 17258-17263, 2004. Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324 Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334 D'Souza CA, et al. Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts. mBio 2: e00342-10, 2011. PubMed: 21304167 Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988

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