| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--19.0, 2.9, 2.5, 2.5, 2.15, 2.0, 1.75, 1.5, 1.25, 1.15, 0.9, 0.6 + smaller; HindIII--23.0, 19.0, 2.8; EcoRI--23.0, 19.5, 2.4, 1.0; BamHI--19.0, 17.0, 5.8, 1.5, 1.4. Codominant segregation was observed in 15 families (260 individuals). Low background using purified insert with usual stringency conditions. Enzyme(s) not detecting polymorphism: ApaI, BglII, EcoRI, HindIII, MspI, PstI, PvuII, SacI, StuI, TaqI, XbaI in 6 unrelated individuals. |
| References |
Griffiths LR, et al. A chromosome 1 BglI RFLP for the LR67 anonymous DNA segment [D1S26]. Nucleic Acids Res. 16: 7752, 1988. PubMed: 2901066
Griffiths LR, et al. Isolation of chromosome 1 probes. Cytogenet. Cell Genet. 46: 623-624, 1987.
Griffiths LR, et al. Isolation and use of chromosome 1 probes for linkage studies on Charcot-Marie-Tooth disease. Ann. Hum. Genet. 54: 31-37, 1990. PubMed: 2321912
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