| Comments |
Restriction digests of the clones give the following sizes (kb): BamHI--15.0, 10.0, 8.6 (doublet), 4.1, 3.1, 2.4, 1.8, 1.0; HindIII--19.0, 15.0, 5.0, 4.5, 3.8, 3.6, 3.5; EcoRI--23.0, 8.6, 6.0, 5.2, 2.6, 2.5, 2.3, 2.1, 0.9. BglII--9.4 (doublet), 6.5, 5.4, 5.1, 3.4, 2.4, 2.3, 1.6, 1.4, 1.35, 1.2, 1.0, 0.7, 0.6; NotI--42.0, 8.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: TaqI, PstI. |
| References |
Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986
Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.199) on chromosome 16 [D16S155]. Nucleic Acids Res. 17: 8020, 1989. PubMed: 2477808
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