Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--7.6; PstI--5.1, 1.1 (doublet); PvuII--3.3 (doublet), 0.7; HindIII--uncut; XbaI--7.6.
The HindIII site has been lost.
Mobilizable cosmid vector.
Media
ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References
Connell TD, et al. A new mobilizable cosmid vector for use in Vibrio cholerae and other gram-negative bacteria. Gene 153: 85-87, 1995. PubMed: 7883191
Shipped
freeze-dried
Shipping Information
?Freeze dried Escherichia coli containing the cosmid.
