| Insert |
DNA: genomic Insert lengths(kb): 17.5 Tissue: lymphocyte Gene product: enolase 3, (beta, muscle)(enolase, beta, muscle-specific) [ENO3] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--uncut; SacI--20.0, 8.8, 4.4, 3.2, 2.1, 1.8, 1.3, 1.0 + smaller; XhoI--20.0, 8.8 (doublet), 7.4, 1.4; XbaI--20.0, 8.8, 7.4, 4.4, 2.1 + smaller; BglII--22.0, 7.0, 5.6, 4.3, 3.2, 1.5; KpnI--23.0, 6.6, 5.2, 3.7, 3.1, 0.61 + smaller. SacI, XbaI and XhoI sites flank the insert. An XhoI digest gives fragments of 8.8, 7.4, and 1.3 kb from the insert. The 8.8 kb fragment bears the entire gene. |
| References |
Peshavaria M, et al. Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17: 8862, 1989. PubMed: 2587223
Ian N Day, personal communication
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