| Vector Information |
Size (kb): 7.0560002326965330 Vector: YCp50-poly (plasmid) Construction: YCp50, pUC19 polylinker Marker(s):URA3,ampR Construct size (kb): 7.056000232696533 Features: marker(s): URA3
marker(s): ampR
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; SmaI--5.1, 2.2; PstI--3.3, 1.8, 1.1, 0.9; PvuI--7.2. YC-type shuttle vector. YCp50-poly was constructed in two steps: (1) YCp50 (ATCC 37419) was cleaved with XhoI and Asp718, blunted and ligated. (2) This construct was then cleaved with EcoRI and SalI, and the EcoRI-PvuII fragment of pUC19 was inserted. |
| References |
Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750
Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414
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