| Comments |
Restriction digests of the clone give the following sizes (kb): AvaI--3.85, 0.69; ClaI--4.4; EcoRI--4.4; SalI--4.4; HindIII--4.4. Contains Sau3A (225), AccI (300), HinfI (345), DdeI (365), and SacII (540) sites (approximate distances in bp from 5' end). Suggest using the AvaI/Sau3A fragment as there are 50 bp of Alu sequences 3' to Sau3A site. NIH 3T3 cells were transformed with DNA from the melanoma NK14 cell line. DNA from secondary transfectants was used to isolate the clone. |
| References |
Padua RA, et al. A novel transforming gene in a human malignant melanoma cell line. Nature 311: 671-673, 1984. PubMed: 6090953
Spurr NK, et al. Chromosomal assignment of c-mel, a human transforming oncogene, to chromosome 19 (p13.2-q13.2). Somatic Cell Mol. Genet. 12: 637-640, 1986. PubMed: 3466361
Rose. Ann Padua, personal communication
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