Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--6.3, 1.45; BglII--7.5; EcoRI--6.2, 1.5. The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 6015 bp BamHI/HindIII fragment of YRp8 containing URA3 was ligated to a 1415 bp BamHI/HindIII fragment of YRp7-32cat (ATCC 37624). |
