| 產(chǎn)品名稱 |
pAS1-CDK2 |
| 商品貨號(hào) |
B236044 |
| Designations |
pAS1-CDK2 |
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Applications |
in another host, produces protein cyclin-dependent kinase 2 |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA-binding domain, ->
epitope tag: hemagglutinin (HA), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDK2
Gene name: cyclin-dependent kinase 2
Contains complete coding sequence?: U
Localization: 12 q13
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb):
Cross references: Gene product: cyclin-dependent kinase 2 [CDK2] Target Gene: cyclin-dependent kinase 2 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): XhoI--7.2, 0.9; EcoRI--8.2; BamHI--8.2; HindIII--4.6, 2.0, 0.9, 0.7; BamHI/HindIII--4.6, 1.8, 0.9, 0.7, 0.2. Used as a negative control in the 2-hybrid cloning system. |
| References |
Elledge SJ, et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907-2911, 1992. PubMed: 1372993
Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751
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