| Vector Information |
Size (kb): 8.9899997711181640 Vector: pMMB278 (plasmid) Promoters: Promoter tac Construction: pMMB190, pMMB311 Marker(s):ampR Construct size (kb): 8.989999771118164 Features: insert detection: lacZ'
marker(s): ampR
promoter: tac
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0; EcoRV--5.4, 3.7; SspI--5.0, 1.7, 1.5, 0.9; EcoRI/MluI--8.2, 0.8. Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Constructed by replacing the MluI/EcoRI fragment of pMMB190 (taclacUV5 promoter) with the MluI/EcoRI fragment of pMMB311 (tac promoter). |
