Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.8, 1.9; EcoRI--2.8, 1.9; HindIII--4.2, 0.56. Codominant segregation of the BglII RFLP observed in 36 3-generation families. PvuII and PstI also appear to reveal polymorphisms, but these have not been verified by pedigree studies. RFLPs observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: EcoRI, BamHI, HindIII. |
