| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--2.5, 1.0; SalI--2.25, 0.95; PstI--2.5, 0.93; EcoRI--2.4, 0.99. The clone was constructed by excising the PstI fragment encoding the full-length cDNA from pHPT30 and inserting the fragment into pUC7. The insert includes about 100 bp 5' untranslated and some 3' untranslated sequence, but not the AATAAA sequence. The insert detects the same BamHI polymorphism detected by the probe, pDSK1. Enzyme(s) not detecting polymorphism: EcoRI, HindIII, MspI, KpnI, TaqI. |
| References |
Jolly DJ, et al. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 80: 477-481, 1983. PubMed: 6300847
Brennand J, et al. Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. J. Biol. Chem. 258: 9593-9596, 1983. PubMed: 6688416
Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107
C. Thomas Caskey, personal communication
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