Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 0.47; BamHI--3.27; PstI--3.27; HindIII--3.27; BglII--3.27. The insert includes the complete coding sequence, extending from 3 bp upstream of the initiation methionine to the end of the transcript, a total of 474 bp. The nucleotide sequences encoding the NH2-terminal leader peptide and first 26 amino acids of GYPA and GYPB are nearly identical. There is also a very homologous region encoding the presumed membrane-spanning portion of the proteins. |
