Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--2.9, 0.56; HindIII--2.8, 0.52; PstI--3.0, 0.3; BglII--3.3; BamHI--3.3. The insert contains the following restriction sites (nt from the 5' end): BamHI--222; BglII--502; HindIII--537; PstI--3, 302. The insert corresponds to nt 63-612 of the sequence record. It contains 37 bp of 5' untranslated region, the complete coding sequence of 324 bp and 189 bp of 3' untranslated region. The insert detects genomic sequences in the rat, hamster and chicken. |
