| Insert |
DNA: genomic Insert lengths(kb): 5.800000190734863 Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8, 4.4 (doublet), 2.3, 0.4; EcoRI/HindIII--4.4 (doublet), 2.3, 1.6, 1.4 (doublet), 1.25, 0.75, 0.4; BamHI--uncut; BglI--11.0, 5.0, 2.4; EcoRV--7.2, 3.9, 3.4, 2.2, 1.0, 0.3; PstI--9.6, 4.3, 2.6, 1.0 (doublet). This plasmid complements the pyrF mutation in E. coli. A 6.2 kb EcoRI fragment contains 377 bp of vector sequence and all of the insert except for 50 bp. The gene is in a 2.8 kb EcoRI/SacI fragment. |
