| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.399, 2.265, 4.747, 1.202, 1.816, 4.232, 2.260, 1.414, 3.064, 3.478, 3.682. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 1.2, 1.4, 1.9, 2.3, 3.2, 3.7, 3.8, 4.3, 5.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70303, 70648, 70649. |
