| 產(chǎn)品名稱(chēng) |
HFBCD05 |
| 商品貨號(hào) |
B237759 |
| Designations |
HFBCD05 |
| GenBank Number |
M77924
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DLAT
Other gene symbols: DLTA
Gene name: dihydrolipoamide S-acetyltransferase (E2 component of
pyruvate dehydrogenase complex)
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 q23.1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M77924; EST: EST01508; Primer: M13 Forward;
THC id: THC121801 Insert lengths(kb): 1.700000047683716 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)( M2 antigen complex 70 kDa subunit, dihydrolipoamide S-acetyltransferase) [DLAT] |
| Insert Size (kb) |
1.700 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9, 0.8; BamHI--4.7; PstI--3.8, 0.7, 0.2. EST01508 matches GenBank accession HUMPDCE2 with 98.43% identity over the sequence range: 1-255. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
