| 產品名稱 |
pcD-MCGF-b9 [moIL-3cDNA] |
| 商品貨號 |
B237766 |
| Designations |
pcD-MCGF-b9 [moIL-3cDNA] |
| GenBank Number |
K01668
|
| Species |
Mus musculus, mouse |
| Depositors |
DNAX Research Institute of Molecular and Cellular Biology, Inc., EP Ching, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications |
contains sequence mast cell growth factor |
| Vector |
Construct size (kb): 3.9499990940094 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il3
Other gene symbols: Mgf
Genomic copy number: unique
Gene name: interleukin 3 (colony-stimulating factor, multiple)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11
Strain: C57BL/6
Tissue: T-cell line Cl.Ly1+ 2-/9, Con A-induced
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 0.95
Cross references: DNA Seq. Acc.: K01668 Insert lengths(kb): 0.949999988079071 Tissue: T-cell line Cl.Ly1+ 2-/9, Con A-induced Gene product: interleukin 3 (colony-stimulating factor, multiple) [Il3] Target Gene: mast cell growth factor |
| Insert Size (kb) |
0.950 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 0.72, 0.45; HindIII--3.6, 0.72; XhoI--2.9, 1.1. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.29; BglII--0.39; HindIII--0.10; XbaI--0.47. Contains the complete coding sequence (nt 28-527). Digestion with XhoI excises the insert plus flanking vector sequence. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Yokota T, et al. Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc. Natl. Acad. Sci. USA 81: 1070-1074, 1984. PubMed: 6322187
Yokota T, et al. cDNA clones coding for polypeptides exhibiting multi-lineage cellular growth factor activity. US Patent 4,695,542 dated Sep 22 1987
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
