The insert includes the following restriction sites (with the PstI site adjacent to the HindIII site of the polylinker = 1): AvaI--151; BamHI--187; PvuII--620; AvaI--711; KpnI--870; EcoRI--876; AccI--1563; EcoRI--1683. Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.7; EcoRI--2.9, 0.8, 0.7; HindIII--4.4. The insert is oriented with the 5' side (3308) closer to the EcoRI site of the polylinker. The insert includes nucleotides 3308 through 5011, encoding part of the coat protein and the overlapping 17K ORF, all of the 50K ORF, and part of the 6.7K ORF. |
