The insert contains the following sites separated by (approximate kb): EcoRI- 2.2- XbaI- 0.6- SstI- 0.7- EcoRI- 0.9- BamHI- 0.25- SphI- 0.55- SstI- 0.1- SphI- 0.7- XbaI. Restriction digests of the clone give the following sizes (kb): 12.0, 4.65, 1.0; BamHI--17.5; SmaI/SalI--7.6 (doublet), 2.25; KpnI--uncut. The HAP3 gene is included in a 1.6 kb BamHI/XbaI fragment. The HAP3 locus encodes 2 overlapping transcripts of 0.57 kb and 4.0 kb read from opposite strands. The smaller mRNA, encoding a protein of 144 aa, directs the synthesis of a protein required for UAS2 activity. |
