Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.98; BglII--3.8; HindIII--3.8; PvuII--2.4, 1.4; BamHI--3.8. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.15; StuI--0.41, 0.67. Nucleotides 23-968 of the insert correspond to nt 2976-3921 of the sequence record. Encodes the carboxy terminal region of the protein and includes part of the 3' untranslated region. One of 4 clones (pI7-11, pF5-1, pA2-10, pA2-13, ATCC 65988-65995) that together contain the complete coding sequence. Cloned in the antisense direction. The 5' EcoRI site was derived from the polylinker. |
