Set of vectors for each of 3 reading frames and a protease-deficient host (WM6, ATCC 47020).
High level of protein expression in protease-deficient hosts (up to 45% of the total soluble protein).
Type Strain
no
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Set of vectors for each of 3 reading frames and a protease-deficient host (WM6, ATCC 47020).
Supports inducible expression from the E. coli trpE promoter.
Fusion proteins contain fewer than 25 amino acids of the TrpE gene product.
High level of protein expression in protease-deficient hosts (up to 45% of the total soluble protein).
Each vector contains the M13 mp13 multiple cloning site.
Product Format
freeze-dried
Depositor
ATCC
References
Rimm DL, Pollard TD. New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli. Gene 75: 323-327, 1989. PubMed: 2653968
