Restriction digests of the clone give the following sizes (kb): BamHI--4.4; KpnI/PvuII--2.6, 1.6; HindIII/PstI--2.8, 1.4. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences. General purpose vector. Constructed by inserting a 0.9 kb PstI fragment from R1 into the PstI site of pUC7, cloning a 0.9 kb BamHI fragment from this intermediate into the BamHI site of pCN38 (ATCC 77095), and deleting a 0.9 kb PstI fragment, to generate the equivalent of pCN38 with part of the multiple cloning site of pUC7. The order of the major features in this plasmid is: PvuII - MCS - kanR - HindIII - KpnI - repA. |
