Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.6; BamHI--3.3, 1.3; HindIII--4.6; PstI--3.5, 1.1, EcoRI/BamHI--2.7, 1.2, 0.5 + smaller. The insert contains the following restriction sites (nt from the 5' end): BamHI--478; EcoRI--595; PstI--673; PvuII--1080. Detects genomic PstI fragments of the following sizes (kb): mouse--6.8, 4.3; Chinese hamster--5.6, 1.9, 0.9. Detects mRNA of approximately 1.9 kb (major) and 4.0 kb (minor) in P815 cells. Transcription from the SP6 promoter gives the sense strand. |
