| Vector Information |
Size (kb): 9.8000001907348630 Vector: YIp1 (plasmid) Construction: pBR322, pGT2-Sc2605 Marker(s):ampR,HIS3 Construct size (kb): 9.800000190734863 Features: marker(s): ampR, HIS3
replicon: pMB1 |
| Comments |
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--9.0, 1.5; EcoRI--9.8. This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock. A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. This was constructed by inserting a 6.1 kb EcoRI/SalI HIS3-containing fragment from pGT2-Sc2605 into pBR322. The HIS3 fragment was originally isolated from the diploid strain A364a X H79-20.3alpha. |
| References |
Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
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