| 產(chǎn)品名稱 |
NAS.4 [NAS-4, YEp24.TRP1ARS.MEL:CMCase, p7] plasmid in E. coli |
| 商品貨號 |
B239160 |
| Designations |
NAS.4 [NAS-4, YEp24.TRP1ARS.MEL:CMCase, p7] plasmid in E. coli |
| Species |
Cellulomonas fimi (McBeth and Scales) Bergey et al. |
| Depositors |
G Strachan |
| Applications |
in another host, produces protein endo-beta-1,4-glucanase |
| Vector |
Construct size (kb): 16.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: YEp24.TRP1.ARS1
Intact vector size:
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites:
Polylinker sites:
Construction: YEp24, YRp7
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3, TRP1
replicon: pMB1, ARS1
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Cellulomonas fimi
Gene name: endo-beta-1,4-glucanase
Contains complete coding sequence?: U
Strain: ATCC 484
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 6.7
Cross references: Insert lengths(kb): 6.7 Gene product: endo-beta-1,4-glucanase |
| Insert Size (kb) |
6.700 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--9.4, 6.7; EcoRI--6.7 (doublet), 2.3; BglII--12.0, 4.0. The vector was constructed by inserting the EcoRI fragment from YRp7 containing TRP1 and ARS1 into the SalI site of YEp24. Constructed by inserting a 2.4 kb BamHI fragment from pEC2.2 (lacking codons for the first 76 aa of cellulase, including the 31 aa signal peptide) into the BglII site of pMEL.D.9.1 (ATCC 53360). An additional codon (AAG, lysine) was present between the terminal codon (GGG, glycine) of the melibiase signal peptide and codon 77 (ATC, isoleucine) of cellulase. ATCC 53361, 53362, and 53363 contain the same 6.7 kb BamHI fragment which comprises the operative melibiase:endoglucanase gene fusion. |
| References |
Skipper NA, et al. DNA sequence useful for the production and secretion from yeast of peptides and proteins. Canadian Patent 1,284,959 dated Jun 18 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
