| 產品名稱 |
pYNB3.1 |
| 商品貨號 |
B239227 |
| Designations |
pYNB3.1 |
| Species |
Homo sapiens, human |
| Depositors |
RL White |
| Vector |
Construct size (kb): 6.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: HincII
Vector end: HincII
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D7S372
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 7; Localization: 7 q35-qter
Type of DNA: genomic
Insert end: RsaI
Insert end: RsaI
Insert size (kb): 4.0
Cross references: Insert lengths(kb): 4.0 Gene product: DNA Segment, single copy [D7S372] Alleles: R1, R2 |
| Insert Size (kb) |
4.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 2.7, 1.3; BamHI--3.0, 1.9, 1.9; EcoRI--6.7; HindIII--5.5, 1.3; PstI--2.8, 1.5, 1.3, 0.8, 0.4. Enzymes(s) not detecting polymorphism: MspI, TaqI. |
| References |
Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220
Raymond L White, personal communication
|
