| 產品名稱 |
28S.3 |
| 商品貨號 |
B239502 |
| Organism |
Ictalurus punctatus, channel catfish |
| Tissue |
peripheral blood |
| Cell Type |
lymphocyte T lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582]. The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
diploid |
| Derivation |
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors.
The cells are immunoglobulin negative [PubMed: 9809582].
The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582]. |
| Comments |
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors.
The cells are immunoglobulin negative [PubMed: 9809582].
The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582]. |
| Complete Growth Medium |
A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5% (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 27.0°C |
| Name of Depositor |
NW Miller |
| Year of Origin |
1996 |
| References |
Wilson MR, et al. T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582
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