| Insert |
DNA: genomic Insert lengths(kb): 15.0 Tissue: liver Gene product: interferon-induced protein 54(interferon, alpha-inducible protein (54kD, ISG-54K, IFI-54K), interferon-stimulated gene, 54KDa protein) [G10P2] |
| Comments |
Insert contains the following sites separated by (bp)(approx): EcoRI- 50- HindIII- 2970- HindIII- 3630- EcoRI- 124- EcoRI- 375- EcoRI- 120- HindIII- 440- EcoRI- 909- EcoRI- 770- HindIII- 1430- EcoRI...(not the complete insert). Restriction digests of the clone give the following sizes (kb): XbaI--19.5, 15.0, 6.4 (doublet); HindIII--21.0, 7.4, 6.0, 5.4 (doublet), 1.0; SalI--uncut; EcoRI--19.5, 10.7, 6.7 (doublet), 3.4; BamHI--28.0, 7.2, 5.8, 4.2, 1.6, 0.45. Region from nt -104 to -90 acts as an interferon-inducible enhancer. Contains the RNA initiation site, exons 1 and 2 plus intervening sequence, and polyA signal of human ISG-54K. The protein predicted from the DNA sequence has a possible limited homology at the amino acid level with murine Mx protein. |
