| 產品名稱 |
pBKV (35-1) |
| 商品貨號 |
B240203 |
| Designations |
pBKV (35-1) |
| GenBank Number |
J02039
|
| Species |
BK polyomavirus, BK Virus |
| Depositors |
PM Howley |
| Vector |
Construct size (kb): 9.30
Name of construct: pBKV (35-1)
Excise insert: BamHI
Size of construct (kb): 9.3
Markers: ampR
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
Genome: BK virus
Contains complete coding sequence?: U
Strain: MM
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: J02039 |
| Insert Size (kb) |
4.963 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
SPECIAL CHARACTERISTICS: ORIGINAL SOURCE: Lysate of infected tissue culture cells. Entire genomic DNA linearized at BamHI site into BamHI site of pBR322 SPECIAL CHARACTERISTICS: The entire viral genome was isolated from an infected human brain. BK virus was linearized with BamHI before cloning. |
| Classification |
Polyomaviridae, Polyomavirus |
| References |
Yang RC, Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science 206: 456-462, 1979. PubMed: 228391
|
