ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt positions according to the published sequence): EcoRI--434; HindIII--227; ScaI--1670.
Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--4.2, 2.2; HindIII--4.1, 2.3; SacI-- uncut; PstI--6.4.
Contains the complete coding sequence (nt 38-852).
pMG-5 is a pBR322 derivative in which the sequence adjacent to the EcoRV site has been modified (GGATATCG to GGATATCC) to allow generation of flanking BamHI sites after homopolymeric tailing EcoRV-cut vector with C's.
References
Gubler U, et al. Recombinant human interleukin 1 alpha: purification and biological characterization. J. Immunol. 136: 2492-2497, 1986. PubMed: 3485152
