ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Frozen glycerol stock of E. coli containing the plasmid
Comments
The insert contains RsaI (5), PstI (1), and XbaI (2) sites.
Restriction digests of the clone give the following sizes (kb): HindIII--4.3, 2.5; PstI--4.0, 2.9; XbaI--6.3, 0.66; XbaI/HindIII--4.3, 1.4, 0.66; XbaI/PstI--4.0, 2.2, 0.66.
This is a subclone of lambda285-1225 (the central 2.55 kb HindIII fragment) and contains 15 repeat monomers.
Classification
Enterobacteriaceae, Escherichia
References
Donlon T, et al. Alpha satellite-like sequences at the centromere of chromosome #8. Am. J. Hum. Genet. 39: A196, 1986.
Choo KH, et al. A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res. 19: 1179-1182, 1991. PubMed: 2030938
