Insert contains an internal PstI site. Restriction digests of the clone give the following sizes (kb): AvaI--2.7, 1.7; EcoRI--4.5; PvuII--4.5; NotI--uncut; SalI--4.5; BamHI--4.5; PstI--3.9, 0.5. This clone corresponds to the alpha form of SAA1 and is completely homologous to alpha apoSAA1. Clone includes 30 bp corresponding to the 5' amino acid leader sequence, about 70 bp of 3' untranslated region and a poly(A)+ tail of about 120 bp. |
