| Insert |
DNA: genomic Insert lengths(kb): 15.0 Tissue: liver Gene product: DNA Segment, single copy [D10S12] Alleles: A1, A2, A3, A5, A6, A4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 15.0, 11.0; SmaI--23.0, 13.5, 7.6; BglII--22.5, 10.0, 5.0, 2.1, 1.8, 1.4; BamHI--15.0, 8.0, 5.8, 4.1, 3.6, 2.9,2.0, 1.5, 1.2, 0.5; HindIII--20.5, 8.0, 5.8, 4.2 (doublet),, 2. The 9.6 kb constant band of the HindIII polymorphism is variable in some families. Enzyme(s) not detecting polymorphism: BamHI, BglII, HincII, RsaI, EcoRI (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Kazazian HH Jr., et al. Characterization of a spontaneous mutation to a B-thalassemia allele. Am. J. Hum. Genet. 38: 860-867, 1986. PubMed: 3014870
Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474
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