Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 13.5, 9.0; EcoRI--20.0, 13.5, 9.0; XhoI--20.0, 13.5, 9.0. Vector permitting positive selection (Spi) of and visual detection (lacZ') of recombinants. Useful for generating jumping and linking libraries. Also permits construction using partial filling-in. The polylinker sites attached to each arm are the same. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7. The order of the major features in this plasmid is: arm - XbaI/MCS/AvrII XbaI - lacZO --> - stuffer - <--lacZO - XbaI AvrII/MCS/XbaI - arm. |
