| 產(chǎn)品名稱 |
AA35 |
| 商品貨號 |
B241167 |
| Designations |
AA35 |
| GenBank Number |
L05929
M91388
|
| Species |
Homo sapiens, human |
| Depositors |
A Swaroop |
| Applications |
contains sequence DNA Segment contains sequence SEC13 (S. cerevisiae) - like 1 |
| Vector |
Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SEC13L1
Other gene symbols: D3S1231E
Gene name: SEC13 (S. cerevisiae) - like 1
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p25-p24
Tissue: retinal pigment epithelial cell line
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI
Insert 3' end: Modification: HindIII
Insert size (kb): 1.322
Cross references: DNA Seq. Acc.: L05929
Nucleotides 45-405 of the insert correspond to
nucleotides 87-447 of L05929 with 61% similarity.
DNA Seq. Acc.: M91388
Nucleotides 1322-1085 of the insert correspond to
nucleotides 1-238 of M91388 with 100% identity. Insert lengths(kb): 1.322000026702881 Tissue: retinal pigment epithelial cell line Gene product: SEC13 (S. cerevisiae) - like 1 [SEC13L1] Target Gene: DNA Segment, SEC13 (S. cerevisiae) - like 1 |
| Insert Size (kb) |
1.322 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 1.3; PvuII--2.5, 0.7, 0.5, 0.45, 0.2; SacI--3.8, 0.5; SalI--3.1, 1.2; SphI--4.3. The insert contains the following restriction sites (approximate kb from the 5' end): BspHI--0.18; HincII--0.16; PvuII--0.48, 0.71, 1.17; SacI--0.38; SalI--0.16; SphI--0.31. Detects a related sequence on human chromosome 18 q11.2-q12. |
| References |
Swaroop A, et al. Molecular characterization of a novel human gene, SEC13R, related to the yeast secretory pathway gene SEC13, and mapping to a conserved linkage group on human chromosome 3p24-p25 and mouse chromosome 6. Hum. Mol. Genet. 3: 1281-1286, 1994. PubMed: 7987303
|
