| Vector Information |
Size (kb): 4.8000001907348630 Vector: pSLF104 (plasmid) Promoters: Promoter adh Marker(s):ampR,sup3-5 Construct size (kb): 4.800000190734863 Features: marker(s): ampR
marker(s): sup3-5
promoter: adh
repressor gene: tet
MCS: HindIII...EcoRI |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.4, 0.58; BamHI--2.9, 1.9; EcoRI--3.6, 1.2. The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter. When integrated into a strain of genotype h- ade6-704 leu1-32 ura4-D18, the resulting fission yeast strain, FY191 (ATCC ) produces a constitutive tetracycline repressor protein. |
