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pEM301 [pHUMDUT1]

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編號:B244341

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產品名稱	pEM301 [pHUMDUT1]
商品貨號	B244341
Designations	pEM301 [pHUMDUT1]
GenBank Number	M89913
Species	Homo sapiens, human
Applications	in another host, produces protein dUTP pyrophosphatase
Vector	Construct size (kb): 3.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTZ19R Intact vector size: 2.900 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ' marker(s): ampR promoter: lac, T7 replicon: pMB1, f1 terminator: none Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DUT* Genomic copy number: multiple Gene name: dUTP pyrophosphatase (dUTPase) Contains complete coding sequence?: Y Chromosome: 15; Localization: 15, cR Type of DNA: cDNA Insert end: Modification: EcoRI adapters Insert end: Modification: EcoRI adapters Insert size (kb): 0.6 Cross references: DNA Seq. Acc.: M89913 Insert lengths(kb): 0.6000000238418579 Gene product: dUTP pyrophosphatase [DUT*] Target Gene: dUTP pyrophosphatase
Insert Size (kb)	0.600
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): HindIII--3.5; XmnI--2.4, 1.1; SmaI--3.3, 0.15; EcoRI--2.9, 0.6; PstI--3.5. The insert contains the following restriction sites (nt from the 5' end): SphI--17, 86; SmaI--134. Contains the complete coding sequence, and corresponds to nt 1-545 of the database record. The sense strand is packaged. pEM301 (ATCC 79032/3) and pEM302 (79034/5) differ only in the orientation of the insert.
References	McIntosh EM, et al. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc. Natl. Acad. Sci. USA 89: 8020-8024, 1992. PubMed: 1325640 Evan M McIntosh, personal communication

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